Identification of 175 Zymogenous Gram negative Bacteria Lines with Numerical Determination Method

Objective To study Gram negative bacteria resistance of the patients in ICU.

目的研究重症监护患者感染革兰阴性杆菌的耐药性，为急危重症患者感染的治疗提供依据。

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Lipopolysaccharide ( LPS) is the major component of the outer membranes of the walls of gram negative bacteria that protects bacterial cells against antibacterial agents.

脂多糖（LPS）是革兰氏阴性细菌外膜的主要组成部分。它能通过对抗抗菌剂来保护细菌的细胞壁。

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All the experiments conducted and their results proved that outer membrane proteins of Gram negative bacteria played a functionally important role during carbohydrate metabolism.

这些结果均显示外膜蛋白在革兰氏阴性细菌的葡萄糖代谢中起到了重要作用。

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The San Marino pit lane has been full of famous names this weekend.

本周末的圣马力诺维修站中挤满了各种各样的名人.

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Book online the cheapest hotels in San Marino-low prices and high discounts.

网上预定在圣马力诺最便宜的酒店 - 价格优惠折扣多多.

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The town in the foreground of the above picture is San Sebastian, Spain.

图片中最显著的是西班牙的圣塞巴斯蒂安.

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Application of Gram-stain in the diagnosis of bacterial vaginosis

革兰染色法在诊断细菌性阴道病中的应用

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Direct immunofluorescence staining technique using Gardnerella monoclonal antibody and Gram stain were used to examine the smears of abnormal vaginal secretion of 247 outpatients in Gynecology Department. Gardnerella vaginitis ( GV) was found in 6 cases ( 2.4%).

应用单克隆抗体直接免疫荧光染色，在妇科门诊247例异常阴道分泌物涂片中检出加德纳菌感染者6例（2.4%），诊断为加德纳杆菌性阴道炎。

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This paper discusses the Gram stain principle in terms of the specific molecular structure and the different ionization state in different PH solutions of the triphenylmethane dye, which will ionize an ion with a positive electric when in the solution with PH higher than 3.2;

从染色剂三苯甲烷特有的分子结构、三苯甲烷染色剂在不同pH溶液中有不同的电离状态，当pH>3.2溶液时它将电离为带一个正电荷的离子的特质；

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This paper separated 5 strains possessing character of lactic acid bacterium from traditional fermented cabbage, named C1~ C5, and identified that those strains are lactic acid bacterium by Gram stain, observing SEM configuration and physiological experiment.

从泡白菜中分离出5个具有乳酸菌菌落特征的菌株，菌株编号分别为C1~C5，经革兰式染色、扫描电子显微镜观察、生理试验等初步鉴定为乳酸菌。

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Conclusion The results suggest that the elevated concentration of endotoxin in BALF> 1: 16 is superior to Gram stain for the rapid identification of patients with gram-negative pneumonia, and this test helps early administration of antimicrobial treatment.

结论结果提示，BALF中内毒素浓度≥1：16快速诊断阴性菌肺炎的准确性明显优于革兰染色检查，该方法有助于早期抗菌药物治疗的选择。

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Scrape the colonies after purification, and prepare to get the bacterial suspension. 2. Identification of Campylobacter jejuni: the gram stain, peroxidase test, hippuric acid hydrolysis test. 3.

鉴定空肠弯曲菌：革兰染色、过氧化物试验、马尿酸水解试验。

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[ Method] All cases from the initial application of CMOS microscope were investigated retrospectively, 30 positive cases were selected respectively to compare with Clearview rapid diagnostic kit, isolating culture and Gram stain smear.

方法回顾性调查该科性病门诊自应用万倍显微镜以来的所有病例，分别选取30例患者与Clearview快速诊断试剂盒检测、分离培养及染色涂片检测结果进行比较。

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The samples were cultured with aerobic, anaerobic and fungus under 37 ℃ for 5 days and 28 ℃ for 7 days, and went through smear, gram stain and microscope detection.

经需、厌氧菌及霉菌培养37℃5d，28℃7d，经涂片、染色、镜检。

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This tissue gram stain of an acute pneumonia demonstrates gram positive cocci that have been eaten by the numerous PMN's exuded into the alveolar space.

图示：革兰氏染色显示大量渗出到肺泡腔的嗜中性粒细胞吞噬了革兰阳性菌。

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The coincidence rate between CMOS microscope and Clearview rapid diagnostic kit, isolating culture, Gram stain smear was all more than 90%.

万倍显微镜与Clearview快速诊断试剂盒检测、分离培养及染色涂片检测的检出符合率达90%以上。

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Methods One sterile swab directly smear; gram stain; bacterioscopy, others swabs inoculate chocolate plate;

方法：宫颈分泌物直接涂片、革兰染色、显微镜检与宫颈分泌物接种于巧克力板、血平板、沙氏板进行培养和鉴定。

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The isolations were identified by gram stain, biochemical reactions and pathogenicity tests in mice.

从10个规模化猪场以直肠棉拭子采集PWD病料，经细菌分离纯化、革兰氏染色、生化试验和小鼠致病性试验等，对病原进行鉴定。

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Semi-nested polymerase chain reaction used for the rapid detection of bacteria and the Gram stain typing and its preliminary applications

快速检测细菌并革兰分型的半巢式聚合酶链反应及其初步应用

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Bacteria in colony were identified with gram stain and PCR method.

细菌通过革兰染色和PCR法鉴定。

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