hkc

Study in altered expression of integrin-linked kinase in Epithelial-mesenchymal transition of HKC induced by MCP-1

MCP-1诱导的肾小管上皮-间充质细胞转化与整合素连接激酶表达变化的关系

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The expression of transforming growth factor β( TGF β 1) in the supernatant of HKC cells was assessed with ELISA.

采用免疫酶标技术（ELISA）检测HKC细胞上清液中转化生长因子β1（TGFβ1）的表达。

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When compared with the curcumin of same concentrations, the growth inhibitory effects of these two prodrugs on HKC cells were significantly decreased ( P0.01).

与同浓度姜黄素比较，两种前体药物对HKC细胞生长的抑制作用均显著降低（P0.01）。

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Methods: Using cell culture and Western blot, the TGF-� � 1-induced expression of FN and ILK was tested.

方法培养人肾小管上皮细胞(HKC),用蛋白印迹方法检测TGF - β1诱导HKC合成 FN和表达ILK的作用.

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Conclusion The cell cycle process of HKC could be inhibited by high concentration of BK with increased apoptosis and an increase in [ Ca~ 2+]_i, which might be the mechanism of inhibition of growth of HKC in vitro.

结论高浓度BK可抑制HKC周期进程、明显促进其凋亡及诱导[Ca2+]i升高，这可能是其使HKC体外生长受抑的部分机制。

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Effect of ILK 、 MMP-9 in High-glucose-induced Tubular Epithelial-myofibroblast Transdifferentiation in HKC

ILK、MMP-9在高糖诱导人肾小管上皮细胞转分化中的表达及意义

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Objective To establish MCP-1 siRNA human renal tubular epithelial cells line and to investigate the inhibitory effect of plasma-mediated small interfering RNA ( siRNA) on the expression of monocyte chemoattractant protein-1 ( MCP-1) gene in human renal tubular epithelial cells ( HKC).

目的建立单核细胞化学吸引蛋白质1（MCP-1）小分子干扰RNA（siRNA）人肾小管上皮细胞株，并检测MCP-1siRNA对人肾小管上皮细胞（HKC）MCP-1基因的抑制效应。

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Objective To test the possible role of monocyte chemotactic protein-1 ( MCP-1) and its synergistic effect with aristolochic acid I ( AA ⅰ) on tubular epithelial-myofibroblast transdifferentiation ( TEMT) of human renal tubular epithelial cells ( HKC) in vitro.

目的探讨单个核细胞趋化蛋白1（MCP1）和马兜铃酸Ⅰ（Aristolochic AcidⅠ，AAⅠ）及其两者的协同作用，对人类肾小管上皮细胞（HKC）转分化的影响。

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Overexpression of ILK in HKC tansfered with pCMV-wtILK could induce FN expression.

转染pCMV-wtILK质粒的HKC细胞,其FN的表达呈增加趋势.

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Methods: PPAR-γ expression in different genotypes of Han: SPRD rat kidney tissues, cyst-lining epithelia, human kidney cortex cell line ( HKC) are studied by RT-PCR and immunohistochemistry.

方法：免疫组化和RT-PCR方法观察PPAR-γ在Han：SPRD大鼠不同表现型肾组织、人近曲小管细胞系（HKC）、囊肿衬里上皮细胞（PKD）中表达分布。

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Methods By using flow cytometer ( FCM) bound with Fluo-3 staining, the effects of histamine and the histamine H_2 receptor antagonist cimetidine on the free intracellular calcium concentration of HKC cultured in vitro, were examined half-quantified.

方法利用流式细胞仪（FCM）结合Fluo3染色技术，半定量检测组胺及H2组胺受体拮抗剂西咪替丁对体外培养HKC胞内Ca2+浓度的影响。

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Furthermore, the epithelial regeneration and HKC differentiation can also be inhibited by BK.

BK还可抑制表皮再生和HKC分化。

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ConclusionThere is an additive effect but no synergism between IGF-I and TGF-� � 1 on EMT.

IGF-I 和TGF - β1对诱导HKC转化无协同作用.

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After treatment with these two prodrugs for 6~ 24 h, the growth inhibition rates on human bladder cancer EJ cells and renal tubular epithelial ( HKC) cells were detected using MTT colorimetry.

MTT比色分析法比较两种姜黄素前体药物作用6~24h后，人膀胱癌EJ细胞及肾小管上皮HKC细胞生长抑制的差异。

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Intergranular corrosion is not found in HKC by using optical microscope.

通过金相观测1Cr18Ni9Ti在HKC中未发现晶间腐蚀。

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Effects of SREBP-1 targeted RNAi on lipid droplet formation in HKC cells under stimulation of high glucose

靶向抑制人SREBP-1基因对高糖刺激下人肾小管上皮细胞脂滴形成的影响

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Objective: To investigate the relationship between TGF-β_1-induced epithelial to myofibroblast transition ( EMT) of human kidney tubular cells ( HKC) and its cellular smad mediated signaling pathway.

目的：研究转化生长因子β1（TGFβ1）诱导人肾小管上皮细胞（HKC）转分化作用与其细胞内信号传递途径的关系。

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ABSTRACT: Objective To investigate whether aristolochic acid can be transported into human kidney proximal tubular cell ( HKC) and its potential mechanism.

摘要：目的探讨马兜铃酸是否能进入人近端肾小管上皮细胞（HKC）及其进入HKC的可能途径。

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Conclusion Histamine can activate correlative signal conduct systems through histamine H_2 receptors on plasma membrane of HKC, and subsequently elevate the free intracellular calcium concentration.

结论组胺通过H2组胺受体激活HKC相关信号传导系统，从而引起细胞内Ca2+浓度增加。

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